{"id":2251,"date":"2009-09-21T13:13:15","date_gmt":"2009-09-21T13:13:15","guid":{"rendered":"http:\/\/catai.net\/blog\/?p=2251"},"modified":"2009-09-23T07:34:24","modified_gmt":"2009-09-23T07:34:24","slug":"patoinformatica-osna-one-step-nucleic-acid-amplification","status":"publish","type":"post","link":"https:\/\/catai.net\/blog\/2009\/09\/patoinformatica-osna-one-step-nucleic-acid-amplification\/","title":{"rendered":"Patoinformatica: OSNA-One Step Nucleic Acid Amplification"},"content":{"rendered":"<p>En el INFORME DE LA CATEDRA de 2009 sobre PATOINFORMATICA, damos cuenta que la patologia esta perdiendo su campo morfologico y adentrandose en soluciones diagnosticas que implican <a href=\"http:\/\/www.patologia.es\/volumen42\/vol42-num2\/42-2n02.htm\">teletrabajo y automatizacion<\/a>.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignleft\" src=\"http:\/\/www.nature.com\/bdj\/journal\/v203\/n9\/images\/bdj.2007.1040-i2.jpg\" alt=\"\" width=\"280\" height=\"207\" \/><a href=\"https:\/\/catai.net\/blog\/wp-content\/uploads\/2009\/09\/dakolink.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-medium wp-image-2306\" title=\"dakolink\" src=\"https:\/\/catai.net\/blog\/wp-content\/uploads\/2009\/09\/dakolink-300x220.jpg\" alt=\"\" width=\"300\" height=\"220\" srcset=\"https:\/\/catai.net\/blog\/wp-content\/uploads\/2009\/09\/dakolink-300x220.jpg 300w, https:\/\/catai.net\/blog\/wp-content\/uploads\/2009\/09\/dakolink-600x441.jpg 600w, https:\/\/catai.net\/blog\/wp-content\/uploads\/2009\/09\/dakolink.jpg 1283w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a>Quedan fuera de contexto los laboratorios multitudinarios con cadenas de tecnicos realizando las tareas para contar con <a href=\"http:\/\/www.milestonemed.com\/histopathology\/index.php?option=com_content&amp;view=article&amp;id=807&amp;Itemid=182\">robots automatizados<\/a>, con cassettes <a href=\"http:\/\/www.sakura.eu\/\">seccionables<\/a>, con\u00a0<a href=\"http:\/\/sakura.ibrochure-online.eu\/en\/small.html\">films<\/a> en vez de cubreobjetos, automatizando las tinciones especiales en red (Artisan\u2122 Link de <a href=\"http:\/\/www.dako.com\/go\/cat2009\/catalog2009_row.pdf\">Dako<\/a>)&#8230;, y asegurando en fin un <a href=\"http:\/\/www.cerner.com\/public\/Cerner_3.asp?id=30881\">control<\/a> de <a href=\"http:\/\/www.kurabo.co.jp\/bio\/English\/product\/as-200.html\">calidad<\/a> , <a href=\"http:\/\/www.slidepath.com\/php\/products-biobank.php\">tracking<\/a> y riesgo, algo parecido a lo que ocurre en los laboratorios clinicos. Al igual que el diagnostico deja de ser cualitativo y una opinion del patologo para transformarse en <a href=\"http:\/\/www1.qiagen.com\/news\/Paxgene.aspx\">cuantitativo<\/a> y objetivo, permitiendo el eRDA (electronic Remote Data Acquisition) . Hasta la <a href=\"http:\/\/www.pathonet.org\/\">docencia<\/a> deja de ser una labor presencial para poderse convertir en <a href=\"http:\/\/www.slidepath.com\/\">virtual<\/a>.<\/p>\n<p>En la entrada anterior vimos como la Biopsia Optica va a sustituir al patologo, aqui comprobamos como la amplificacion genetica es mas segura y rapida (30 min) que la morfologia en la deteccion de metastasis en el ganglio centinela. Es la tecnica de <a href=\"http:\/\/www.sysmex-lifescience.com\/Anlisis-del-ganglio-centinela-174-4.html\">OSNA<\/a> o\u00a0<strong>O<\/strong>ne <strong>S<\/strong>tep <strong>N<\/strong>ucleic Acid  <strong>A<\/strong>mplification, con amplificaci\u00f3n isot\u00e9rmica RT-LAMP (reverse transcriptase loop-mediated isothermal amplification). La tecnica utiliza un instrumento con <strong>marca  IVD-CE<\/strong>, de acuerdo con la Directiva IVD 98\/79\/EC. El\u00a0RD-100i\u00a0puede ser comercializado  libremente en el Espacio Econ\u00f3mico Europeo (EEA), incluyendo los 27 estados  miembros de la EU-27, para el <strong>diagn\u00f3stico In Vitro <\/strong>de muestras humanas. Se utiliza para detectar el\u00a0ARNm del CK19 como marcador de metastasis ganglionares de mama.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"content alignleft\" src=\"http:\/\/www.sysmex-lifescience.com\/content\/alt\/illu_molecular-background.jpg\" alt=\"...\" width=\"437\" height=\"328\" \/><\/p>\n<p>Ademas podemos predecir mediante el\u00a0<a href=\"http:\/\/www.sysmex-lifescience.com\/Bases-moleculares-183-4.html\">analisis del ciclo celular<\/a> la agresividad del tumor, algo parecido a la telecuantificacion de DNA en la que fuimos los <strong><a href=\"http:\/\/www.telemed.co.rs\/NOVI\/istorijat_01.htm\">primeros en el mundo<\/a> <\/strong>(<a href=\"http:\/\/www.repatologia.com\/crearficheros\/1991\/1991_03.pdf\">Patologia<\/a> (1991) <a href=\"http:\/\/www.teide.net\/catai\/pubs\/videophonePathology.pdf\">vol 24 n3:225-229<\/a>) . Pero lo que es mas importante, su sensibilidad al tratamiento.<\/p>\n<p>Hemos de re-inventar la especialidad y tomar en consideracion el teletrabajo y la telepatologia.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>En el INFORME DE LA CATEDRA de 2009 sobre PATOINFORMATICA, damos cuenta que la patologia esta perdiendo su campo morfologico y adentrandose en soluciones diagnosticas que implican teletrabajo y automatizacion. Quedan fuera de contexto los laboratorios multitudinarios con cadenas de tecnicos realizando las tareas para contar con robots automatizados, con cassettes seccionables, con\u00a0films en vez [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"give_campaign_id":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_hide_featured_image":false,"_jetpack_featured_image_duplicate_dismissed":0,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":false,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[4,11,3,13,28],"tags":[],"class_list":["post-2251","post","type-post","status-publish","format-standard","hentry","category-actividades","category-diagnostico-no-invasivo","category-informes","category-phealth","category-sanidad"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Patoinformatica: OSNA-One Step Nucleic Acid Amplification | Telemedicina<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/catai.net\/blog\/2009\/09\/patoinformatica-osna-one-step-nucleic-acid-amplification\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Patoinformatica: OSNA-One Step Nucleic Acid Amplification | Telemedicina\" \/>\n<meta property=\"og:description\" content=\"En el INFORME DE LA CATEDRA de 2009 sobre PATOINFORMATICA, damos cuenta que la patologia esta perdiendo su campo morfologico y adentrandose en soluciones diagnosticas que implican teletrabajo y automatizacion. 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