{"id":10646,"date":"2016-03-17T09:47:51","date_gmt":"2016-03-17T08:47:51","guid":{"rendered":"http:\/\/catai.net\/blog\/?p=10646"},"modified":"2016-03-17T09:47:51","modified_gmt":"2016-03-17T08:47:51","slug":"luminescence-resonance-energy-transfer-lret","status":"publish","type":"post","link":"https:\/\/catai.net\/blog\/2016\/03\/luminescence-resonance-energy-transfer-lret\/","title":{"rendered":"Luminescence resonance energy transfer (LRET)"},"content":{"rendered":"<p>A technique that in\u00a0a matter of hours can make virus diagnosis reducing\u00a0the cost of detectors by 80%.\u00a0The\u00a0<a href=\"http:\/\/futurism.com\/new-biosensor-rapidly-detects-multiple-viruses-80-cheaper-current-methods\/www.polyu.edu.hk\/web\/en\/home\/index.html\">Hong Kong Polytechnic University<\/a>\u00a0(PolyU) employs a <a href=\"http:\/\/futurism.com\/new-biosensor-rapidly-detects-multiple-viruses-80-cheaper-current-methods\/futurism.com\/nanoparticles-vs-bacteria-researches-developed-photexcited-nanoparticles-that-could-kill-antibiotic-resistant-bacteria\/\">nanoparticle<\/a> biosensor that uses the<span style=\"color: #ff0000;\"><strong> upconversion LRET<\/strong><\/span> to detect viruses in a quick and cheap method, instead of using genetic analysis that is complicated, time consuming and expensive.\u00a0RT-PCR (reverse transcription-polymerase chain reaction)\u00a0is expensive and time-consuming while ELISA has a\u00a0relatively low\u00a0sensitivity.<\/p>\n<div class=\"post-container\">\n<div class=\"ajax-post link\">\n<div class=\"container-fluid content-container\">\n<section class=\"section blog-content\">\n<div class=\"table\">\n<div class=\"col-left\">\n<div class=\"post-content module\">\n<div class=\"summary module\">\n<p>\u00ad<\/p>\n<p><script src=\"http:\/\/b.scorecardresearch.com\/beacon.js\" async=\"\" type=\"mce-mce-mce-no\/type\"><\/script><script id=\"facebook-jssdk\" src=\"\/\/connect.facebook.net\/en_US\/sdk.js#xfbml=1&amp;version=v2.3\" type=\"mce-mce-mce-no\/type\"><\/script><script src=\"https:\/\/d24n15hnbwhuhn.cloudfront.net\/libs\/amplitude-2.4.0-min.gz.js\" async=\"\" type=\"mce-mce-mce-text\/javascript\"><\/script><script src=\"\/\/www.google-analytics.com\/analytics.js\" async=\"\" type=\"mce-mce-mce-no\/type\"><\/script><script type=\"mce-mce-mce-text\/javascript\">\/\/ <![CDATA[\nvar _sf_startpt=(new Date()).getTime()\n\/\/ ]]><\/script><!-- For Naytev --> <!--<meta property=\"og:url\" content=\"http:\/\/social.futurism.com\/new-biosensor-rapidly-detects-multiple-viruses-80-cheaper-current-methods\/\"\/>--> <!-- This site is optimized with the Yoast SEO plugin v3.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ --> <!-- \/ Yoast SEO plugin. --><script type=\"mce-mce-mce-text\/javascript\">\/\/ <![CDATA[\nwindow._wpemojiSettings = {\"baseUrl\":\"https:\\\/\\\/s.w.org\\\/images\\\/core\\\/emoji\\\/72x72\\\/\",\"ext\":\".png\",\"source\":{\"concatemoji\":\"http:\\\/\\\/futurism.com\\\/wp-includes\\\/js\\\/wp-emoji-release.min.js?ver=4.4.2\"}}; 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Given the complementary nature of the DNA base pairs of the UCNPs probe oligo and AuNPs flu virus oligo, they work like two matching pieces of magnet, which would be drawn together due to attraction force. This process is also called <span style=\"color: #ff0000;\"><strong>oligo hybridization<\/strong><\/span>.\u00a0When the sample is then illuminated by <strong>near-infrared light <\/strong>(NIR), the upconversion nanoparticles emit a green radiance, while the AuNPs absorb green light. The concentration of virus is calculated by\u00a0the decrease of green light.<\/p>\n<p>This\u00a0new technique is very simple, without requiring expensive apparatus or equipment to operate, and doesn\u2019t demand any particularly sophisticated user skill-set, with comparable sensitivity to more expensive, traditional methods.\u00a0In comparison to conventional downconversion luminescent technique, it causes low damage to genetic materials and does not induce background fluorescence.<\/p>\n<p>CHIP &amp; ADAPTED to developing countries. In spite of the low cost it is still HK$20.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>A technique that in\u00a0a matter of hours can make virus diagnosis reducing\u00a0the cost of detectors by 80%.\u00a0The\u00a0Hong Kong Polytechnic University\u00a0(PolyU) employs a nanoparticle biosensor that uses the upconversion LRET to detect viruses in a quick and cheap method, instead of using genetic analysis that is complicated, time consuming and expensive.\u00a0RT-PCR (reverse transcription-polymerase chain reaction)\u00a0is expensive [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"give_campaign_id":0,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_hide_featured_image":false,"_jetpack_featured_image_duplicate_dismissed":0,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":false,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[94,43,3,40,13,24,66,28,29,32,9],"tags":[],"class_list":["post-10646","post","type-post","status-publish","format-standard","hentry","category-diy","category-health-4-0","category-informes","category-innovacion","category-phealth","category-productividad","category-reverse-innovation","category-sanidad","category-seguridad","category-sociedad_red","category-unesco"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.8 - 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